Abstract
We have isolated distinct clones for cellular proteins that bind to the retinoblastoma protein by direct screening of cDNA expression libraries using purified pRB as a probe. The total nucleotide sequence of one of these clones, RBQ-3, was determined and found to encode a protein of 66 kDa localized in the nucleus. The RBQ-3 preferentially binds to underphosphorylated pRB. The region used for binding to this protein was mapped to the E1A-binding pocket B of pRB, which has sequence similarity to the general transcription factor TFIIB. We have mapped the gene to 1q32 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism
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Chromosome Mapping*
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Cloning, Molecular
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Cricetinae
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DNA Primers / genetics
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DNA, Complementary / genetics
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Humans
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Hybrid Cells
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Nuclear Proteins / isolation & purification
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Nuclear Proteins / metabolism
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Phosphorylation
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Protein Binding
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Retinoblastoma Protein / genetics*
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Retinoblastoma Protein / isolation & purification
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Retinoblastoma Protein / metabolism
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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DNA Primers
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DNA, Complementary
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Nuclear Proteins
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Retinoblastoma Protein