Enhancing the avidity of a human recombinant anti-HIV-1 monoclonal antibody through oligomerization

J Pharm Sci. 1995 Jul;84(7):866-70. doi: 10.1002/jps.2600840716.

Abstract

The oligomerization by chemical cross-linking of a recombinant human antiviral monoclonal antibody (MAb), r447-1, and its characterization are described. This MAb binds to an epitope residing in the hypervariable V3 region of the envelope protein (gp120/160) of HIV-1. A dimeric form of this MAb displays enhanced avidity and was found to be capable of neutralizing a greater variety of lymphoid cell culture-adapted HIV-1 variants and HIV-1 primary isolates than its monomeric form. The superior binding and breadth of reactivity of this antibody suggests it may have utility as a therapeutic and/or prophylactic agent, if it possesses an appropriate safety and immunogenicity profile.

MeSH terms

  • Antibodies, Monoclonal / genetics*
  • Antigens / immunology
  • Chromatography
  • HIV-1 / immunology*
  • Humans
  • Molecular Structure
  • Proteins / metabolism
  • Recombination, Genetic
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Proteins