Transmission blocking immunity as observed in a feeder system and serological reactivity to Pfs 48/45 and Pfs230 in field sera

W Roeffen; T Lensen; B Mulder; K Teelen; R Sauerwein; W Eling; J H Meuwissen; P Beckers

doi:10.1590/s0074-02761994000600004

Transmission blocking immunity as observed in a feeder system and serological reactivity to Pfs 48/45 and Pfs230 in field sera

Mem Inst Oswaldo Cruz. 1994:89 Suppl 2:13-5. doi: 10.1590/s0074-02761994000600004.

Authors

W Roeffen¹, T Lensen, B Mulder, K Teelen, R Sauerwein, W Eling, J H Meuwissen, P Beckers

Affiliation

¹ University of Nijmegen, Department of Medical Microbiology, The Netherlands.

PMID: 7565122
DOI: 10.1590/s0074-02761994000600004

Abstract

Monoclonal antibodies (mAbs) and human sera from gametocyte carriers were applied in the bio-assay to test for their transmission-blocking capacity. Competition ELISA's have been developed for the detection of natural transmission blocking antibodies. Approximately 55% of the sera blocking in the bio-assay gave positive results in these competition ELISA's.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antibodies, Protozoan / immunology*
Antigens, Surface / immunology*
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Immune Sera / immunology*
Malaria, Falciparum / immunology*

Substances

Antibodies, Protozoan
Antigens, Surface
Immune Sera