Automated linkage analysis in psychiatric disorders

Am J Med Genet. 1995 Jun 19;60(3):192-8. doi: 10.1002/ajmg.1320600305.

Abstract

A genome-wide search for linkage of microsatellite markers to chromosomal loci containing genes responsible for the major psychoses is a laborious task which can be carried out with greater speed and economy by introducing automation to several steps in the procedure. We describe the use of the Automated Linkage Preprocessor (ALP) program for the computer analysis of the waveform generated by fluorescein-labelled markers after electrophoretic separation. (To obtain a copy send a request to A.F. Brown at the below MRC address or use Anonymous FTP to ftp.hgu.mrc.ac.uk. Software is in directory pub/ALP). The program runs on a PC in the Microsoft Windows environment, and is used in conjunction with an automated laser fluorescence (ALF) sequencer (Pharmacia) and its Fragment Manager software to detect and size the PCR products, filter out peaks of fluorescence due to nonallele fragments, and generate genotypes in a format suitable for direct input to standard linkage analysis programs. The method should offer the advantages of speed, accuracy, and reduced cost. Its use in linkage studies in a large family with manic-depressive illness is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation
  • Chromosome Mapping
  • DNA / analysis*
  • Genetic Linkage
  • Humans
  • Psychotic Disorders / genetics*
  • Software*

Substances

  • DNA