Abstract
A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction allowed us to detect and enumerate < or = 10 CFU of C. perfringens per g.
MeSH terms
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Animals
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Base Sequence
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Cattle
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Clostridium perfringens / genetics
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Clostridium perfringens / isolation & purification*
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Clostridium perfringens / metabolism*
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Colony Count, Microbial / methods*
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DNA Probes / genetics
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DNA, Bacterial / genetics
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Enterotoxins / biosynthesis*
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Enterotoxins / genetics
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Evaluation Studies as Topic
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Genes, Bacterial
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Meat / microbiology*
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Molecular Sequence Data
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Polymerase Chain Reaction
Substances
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DNA Probes
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DNA, Bacterial
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Enterotoxins
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enterotoxin, Clostridium