Abstract
The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing protein implicated in the activation of thyroid-specific gene expression. Here we report that TTF-1 is capable of activating transcription from thyroglobulin and, to a lesser extent, thyroperoxidase gene promoters in nonthyroid cells. Full transcriptional activation of the thyroglobulin promoter by TTF-1 requires the presence of at least two TTF-1 binding sites. TTF-1 activates transcription via two functionally redundant transcriptional activation domains that as suggested by competition experiments, could use a common intermediary factor.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cell Line
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Gene Expression Regulation
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HeLa Cells
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / metabolism*
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Humans
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Molecular Sequence Data
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Mutagenesis, Insertional
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / chemistry*
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Nuclear Proteins / metabolism*
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Oligodeoxyribonucleotides
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Promoter Regions, Genetic*
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Rats
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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TATA Box
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Thyroglobulin / biosynthesis*
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Thyroglobulin / genetics
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Thyroid Gland / metabolism*
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Thyroid Nuclear Factor 1
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Transcription Factors / biosynthesis
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Transcription Factors / chemistry*
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Transcription Factors / metabolism*
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Transcription, Genetic*
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Transcriptional Activation
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Transfection
Substances
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Homeodomain Proteins
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NKX2-1 protein, human
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Nkx2-1 protein, rat
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Recombinant Fusion Proteins
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Recombinant Proteins
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Thyroid Nuclear Factor 1
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Transcription Factors
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Thyroglobulin