Cytoadherence-related homologous motifs in Plasmodium falciparum antigen Pf155/RESA and erythrocyte band 3 protein

Parasitology. 1995 Jun:110 ( Pt 5):503-11. doi: 10.1017/s0031182000065215.

Abstract

Cytoadherence of Plasmodium falciparum-infected erythrocytes plays an important role in the pathogenesis of cerebral malaria. The identity of cell surface molecules on parasitized erythrocytes involved in cytoadherence is of great interest to understand the molecular basis of this mechanism. Peptide sequences derived from exofacial loops of the erythrocyte antigen band 3 from parasitized erythrocytes have previously been shown to inhibit cytoadherence. We now report that a non-repeated region of Pf155/RESA (residues 213-218) contains a hexapeptide motif being highly homologous to cytoadherence inhibitory sequences from band 3. Synthetic peptides containing the hexapeptide motif of Pf155/RESA inhibited the binding of P. falciparum-infected erythrocytes to melanoma cells in vitro. Furthermore, individuals residing in malaria-endemic areas have antibodies reactive with epitopes involving these motifs in band 3 and in Pf155/RESA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anion Exchange Protein 1, Erythrocyte / genetics*
  • Anion Exchange Protein 1, Erythrocyte / immunology
  • Antigens, Protozoan / genetics*
  • Antigens, Protozoan / immunology
  • Cell Adhesion
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Humans
  • Malaria, Falciparum / genetics
  • Malaria, Falciparum / immunology
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / immunology
  • Sequence Homology, Amino Acid

Substances

  • Anion Exchange Protein 1, Erythrocyte
  • Antigens, Protozoan
  • Cell Adhesion Molecules
  • Peptide Fragments
  • Protozoan Proteins
  • ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum