Analysis of the iron-sulfur clusters within the complex I (NADH:ubiquinone oxidoreductase) isolated from potato tuber mitochondria

Eur J Biochem. 1995 Jun 15;230(3):1032-6. doi: 10.1111/j.1432-1033.1995.tb20652.x.

Abstract

The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Electron Spin Resonance Spectroscopy
  • Electron Transport Complex I
  • Iron / analysis*
  • Mitochondria / enzymology*
  • NADH, NADPH Oxidoreductases / analysis*
  • NADH, NADPH Oxidoreductases / isolation & purification
  • Solanum tuberosum / enzymology*
  • Sulfur / analysis*

Substances

  • Sulfur
  • Iron
  • NADH, NADPH Oxidoreductases
  • Electron Transport Complex I