Characterisation of an antibody relevant to the neuropathology of Alzheimer disease

Alzheimer Dis Assoc Disord. 1995 Spring;9(1):47-51. doi: 10.1097/00002093-199505000-00009.

Abstract

The specificity of BR88, a polyclonal antibody we raised against amino acids 1-12 of beta/A4 (beta-amyloid protein), has been reexamined in view of a claim made that it cross-reacts with tau-protein, probably the sole component of the paired helical filaments of Alzheimer disease. We have used enzyme-linked immunosorbent assays, immunoblots, and immunohistochemistry, together with appropriate controls, to show that BR88 does not cross-react with tau. This contradicts the claim in question and confirms our previous findings in which BR88 was used to show that some tangle-bearing cells displayed the N-terminus of beta/A4 on their surfaces. The conclusions formed in that earlier work therefore remain intact.

MeSH terms

  • Adult
  • Alzheimer Disease / immunology*
  • Alzheimer Disease / pathology
  • Amino Acids / immunology
  • Amyloid beta-Protein Precursor / immunology*
  • Antibodies / immunology*
  • Antibody Specificity / immunology
  • Brain / immunology*
  • Brain / pathology
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Immunoenzyme Techniques
  • tau Proteins / immunology

Substances

  • Amino Acids
  • Amyloid beta-Protein Precursor
  • Antibodies
  • tau Proteins