Abstract
Lipomas are one of the most common mesenchymal neoplasms in humans. They are characterized by consistent cytogenetic aberrations involving chromosome 12 in bands q14-15. Interestingly, this region is also the site of rearrangement for other mesenchymally derived tumors. This study demonstrates that HMGI-C, an architectural factor that functions in transcriptional regulation, has been disrupted by rearrangement at the 12q14-15 chromosomal breakpoint in lipomas. Chimeric transcripts were isolated from two lipomas in which HMGI-C DNA-binding domains (AT hook motifs) are fused to either a LIM or an acidic transactivation domain. These results, identifying a gene rearranged in a benign neoplastic process that does not proceed to a malignancy, suggest a role for HMGI-C in adipogenesis and mesenchyme differentiation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 12
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Cloning, Molecular
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DNA, Neoplasm / genetics*
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DNA, Neoplasm / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / physiology
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Gene Expression Regulation, Neoplastic / genetics*
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Gene Rearrangement / genetics*
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HMGA2 Protein
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High Mobility Group Proteins / genetics*
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High Mobility Group Proteins / physiology
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Humans
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Lipoma / chemistry
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Lipoma / genetics*
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Mesoderm
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Molecular Sequence Data
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RNA, Messenger / analysis
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RNA, Neoplasm / analysis
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Transcription, Genetic / genetics
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Transcriptional Activation
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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HMGA2 Protein
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High Mobility Group Proteins
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RNA, Messenger
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RNA, Neoplasm
Associated data
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GENBANK/U28131
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GENBANK/U28132