Conditioned medium from a cell strain derived from a patient with mastocytosis induces the development of mature human mast cells in vitro from normal human bone marrow

L Li; J J Macpherson; S Adelstein; C L Bunn; K Atkinson; K Phadke; S A Krilis

doi:10.1159/000236957

Conditioned medium from a cell strain derived from a patient with mastocytosis induces the development of mature human mast cells in vitro from normal human bone marrow

Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):142-4. doi: 10.1159/000236957.

Authors

L Li¹, J J Macpherson, S Adelstein, C L Bunn, K Atkinson, K Phadke, S A Krilis

Affiliation

¹ Department of Immunology, Allergy and Infectious Disease, St. George Hospital, Kogarah, Australia.

PMID: 7613121
DOI: 10.1159/000236957

Abstract

Mature human mast cells were obtained from human bone marrow in a coculture system using conditioned medium from a human mastocytosis cell strain. These cells expressed the high-affinity IgE receptor and were tryptase, chymase and carboxypeptidase positive.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers
Bone Marrow Cells*
Carboxypeptidases / analysis
Cells, Cultured
Chymases
Culture Media, Conditioned / pharmacology*
Humans
Mast Cells / cytology
Mast Cells / drug effects*
Mastocytosis / pathology*
Receptors, IgE / biosynthesis
Receptors, IgE / genetics
Serine Endopeptidases / analysis
Tryptases

Substances

Biomarkers
Culture Media, Conditioned
Receptors, IgE
Carboxypeptidases
Serine Endopeptidases
chymase 2
Chymases
Tryptases