Abstract
We have cloned two inwardly rectifying K+ channels that occur selectively in neurons in the brain and are designated BIRK (brain inwardly rectifying K+) channels. BIRK1 mRNA is extremely abundant and is enriched in specific brainstem nuclei, BIRK1 displays a consensus phosphate-binding loop, and expression in Xenopus oocytes has shown that its conductance is inhibited by ATP and adenosine 5'-[gamma-thio]triphosphate. BIRK2 is far less abundant and is selectively localized in telencephalic neurons. BIRK2 has a consensus sequence for cAMP-dependent phosphorylation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / pharmacology
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Brain / anatomy & histology
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Brain Stem / chemistry*
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Cell Compartmentation
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Cell Nucleus / chemistry
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Cloning, Molecular
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Electric Conductivity
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In Situ Hybridization
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Membrane Potentials / drug effects
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Models, Molecular
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Molecular Sequence Data
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Potassium / metabolism
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Potassium Channels / biosynthesis
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Potassium Channels / genetics*
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Potassium Channels, Inwardly Rectifying*
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RNA, Messenger / analysis
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Rats
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Recombinant Proteins / biosynthesis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Telencephalon / chemistry*
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Tissue Distribution
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Xenopus
Substances
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Potassium Channels
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Potassium Channels, Inwardly Rectifying
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RNA, Messenger
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Recombinant Proteins
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Adenosine Triphosphate
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Potassium
Associated data
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GENBANK/U27558
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GENBANK/U27582