Abstract
Characterization of a ganciclovir-resistant cytomegalovirus strain from a patient with AIDS showed a histidine-to-glutamine change at residue 520 of UL97 (Q520 mutation). In anabolism studies, Q520 was associated with impaired phosphorylation of ganciclovir. Transfer of Q520 to a recombinant virus resulted in a ganciclovir-resistant phenotype.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AIDS-Related Opportunistic Infections / microbiology
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Base Sequence
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Cytomegalovirus / drug effects*
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Cytomegalovirus / genetics*
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Drug Resistance, Microbial
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Ganciclovir / pharmacology*
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Glutamine / metabolism
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Histidine / metabolism
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Humans
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Molecular Sequence Data
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Mutation / genetics
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Phenotype
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Polymerase Chain Reaction
Substances
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Glutamine
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Histidine
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Ganciclovir