Abstract
The DNA-activated serine/threonine protein kinase (DNA-PK) is composed of a large (approximately 460 kDa) catalytic polypeptide (DNA-PKcs) and Ku, a heterodimeric DNA-binding component (p70/p80) that targets DNA-PKcs to DNA. A 41-kbp segment of the DNA-PKcs gene was isolated, and a 7902-bp segment was sequenced. The sequence contains a polymorphic Pvu II restriction enzyme site, and comparing the sequence with that of the cDNA revealed the positions of nine exons. The DNA-PKcs gene was mapped to band q11 of chromosome 8 by in situ hybridization. This location is coincident with that of XRCC7, the gene that complements the DNA double-strand break repair and V(D)J recombination defects (where V is variable, D is diversity, and J is joining) of hamster V3 and murine severe combined immunodeficient (scid) cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Binding Sites / genetics
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Catalysis
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Chromosome Banding
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Chromosome Mapping
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Chromosomes, Human, Pair 8* / ultrastructure
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Cricetinae
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA-Activated Protein Kinase
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DNA-Binding Proteins*
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Exons
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Mice
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Molecular Sequence Data
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Nuclear Proteins
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Protein Conformation
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics*
Substances
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DNA Primers
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DNA, Complementary
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DNA-Binding Proteins
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Nuclear Proteins
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DNA-Activated Protein Kinase
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PRKDC protein, human
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Protein Serine-Threonine Kinases