A relatively small 5' regulatory region of esterase S gene of Drosophila virilis determines the specific expression as revealed in transgenic experiments

L I Korochkin; V M Panin; G V Pavlova; M R Kopantseva; N G Shostak; V N Bashkirov; L B Gabitova; P V Sergeev

doi:10.1006/bbrc.1995.2130

A relatively small 5' regulatory region of esterase S gene of Drosophila virilis determines the specific expression as revealed in transgenic experiments

Biochem Biophys Res Commun. 1995 Aug 4;213(1):302-10. doi: 10.1006/bbrc.1995.2130.

Authors

L I Korochkin¹, V M Panin, G V Pavlova, M R Kopantseva, N G Shostak, V N Bashkirov, L B Gabitova, P V Sergeev

Affiliation

¹ Institute of Gene Biology, Russian Academy of Sciences, Moscow.

PMID: 7639749
DOI: 10.1006/bbrc.1995.2130

Abstract

Expression of the esterase S gene of Drosophila virilis was studied in transgenic experiments. Truncated genomic copy of this gene including 400 bp of 5' regulatory region was integrated into the genome of Drosophila melanogaster. The products of the transferred gene were detected. It was found that strict temporal and tissue specificity of the esterase S gene expression is conserved in transformed flies. The results suggest that this specificity is evidently determined by the regulatory region of the esterase S gene and controlled by cis mechanism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
Carboxylesterase
Carboxylic Ester Hydrolases / analysis
Carboxylic Ester Hydrolases / biosynthesis*
Carboxylic Ester Hydrolases / genetics*
Chromosome Mapping
DNA Probes
Drosophila / enzymology*
Drosophila / genetics
Drosophila Proteins*
Drosophila melanogaster
Gene Expression Regulation, Enzymologic
Gene Expression*
Genes, Insect*
Immunohistochemistry
In Situ Hybridization
Regulatory Sequences, Nucleic Acid*
Restriction Mapping

Substances

DNA Probes
Drosophila Proteins
Carboxylic Ester Hydrolases
Carboxylesterase
esterase-S, Drosophila