Abstract
A simple, rapid and reliable outline for identification of clostridia isolates from human infections was developed. It consists of a combination of API ZYM and API LRA Oxidase tests. The enzymatic activities were performed with strains sub-cultured onto carbohydrate-free medium (Columbia blood agar). Fifty-five strains of Clostridium difficile, C. bifermentans, C. sordellii, and C. perfringens from clinical specimens and eight reference standard strains representing different species of the same genus were analyzed. The accuracy of the new method was evaluated by comparison with the results obtained by DNA/DNA analysis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques*
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Chromosomes, Bacterial / chemistry
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Clostridioides difficile / classification
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Clostridioides difficile / enzymology
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Clostridioides difficile / genetics
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Clostridioides difficile / isolation & purification
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Clostridium / classification*
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Clostridium / enzymology
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Clostridium / genetics
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Clostridium / isolation & purification
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Clostridium Infections / diagnosis
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Clostridium Infections / microbiology*
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Clostridium perfringens / classification
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Clostridium perfringens / enzymology
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Clostridium perfringens / genetics
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Clostridium perfringens / isolation & purification
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DNA, Bacterial / analysis
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Diarrhea / microbiology*
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Feces / microbiology*
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Humans
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Microbiological Techniques
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Oxidoreductases / analysis*
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Reproducibility of Results
Substances
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DNA, Bacterial
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Oxidoreductases