Abstract
Vulval induction during Caenorhabditis elegans development is mediated by LET-23, a homolog of the mammalian epidermal growth factor receptor tyrosine kinase. The sli-1 gene is a negative regulator of LET-23 and is shown here to encode a protein similar to c-Cbl, a mammalian proto-oncoprotein. SLI-1 and c-Cbl share approximately 55 percent amino acid identity over a stretch of 390 residues, which includes a C3HC4 zinc-binding motif known as the RING finger, and multiple consensus binding sites for Src homology 3 (SH3) domains. SLI-1 and c-Cbl may define a new class of proteins that modify receptor tyrosine kinase-mediated signal transduction.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans Proteins*
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Conserved Sequence
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DNA, Complementary / genetics
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ErbB Receptors / metabolism
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Female
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Genes, Helminth*
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Genes, Regulator*
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Helminth Proteins / chemistry
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Helminth Proteins / genetics*
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Helminth Proteins / metabolism
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Humans
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Molecular Sequence Data
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Mutation
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / chemistry
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-cbl
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Sequence Alignment
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Signal Transduction
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Ubiquitin-Protein Ligases*
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Vulva / growth & development
Substances
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Caenorhabditis elegans Proteins
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DNA, Complementary
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Helminth Proteins
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Sem-5 protein, C elegans
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Sli-1 protein, C elegans
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Proto-Oncogene Proteins c-cbl
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Ubiquitin-Protein Ligases
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ErbB Receptors
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let-23 protein, C elegans
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CBL protein, human