[Organ regeneration and somatic embryogenesis from young stems of Fritillaria sinica]

Hua Xi Yi Ke Da Xue Xue Bao. 1995 Mar;26(1):33-7.
[Article in Chinese]

Abstract

Regenerated bulbs and embryogenetic calli were inducted from young stems of Fritillaria sinica cultured on Murashige and Skoog (MS) medium supplemented with alpha-naphthale-neacetic acid (NAA), 2, 4-dichlophenexy-acetin acid (2, 4-D), 6-benzylaminopurine (6BA) and kenetin (Kt) respectively. The callus cultures used for the organ regeneration and somatic embryogenesis were subcultured at intervals of 30-40 days on MS medium+NAA 1mg/L + 6BA 0.5mg/L. In general, the bulbs can be obtained from cultured Fritillaria sinica through three ways: (1) induction from explants; (2) production of adventitious buds or (3) somatic embryogenesis.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Culture Techniques / methods
  • Plants, Medicinal / growth & development*

Substances

  • Culture Media