Gene cloning and complete nucleotide sequence of philippine Schistosoma japonicum paramyosin

Acta Trop. 1995 May;59(2):143-7. doi: 10.1016/0001-706x(94)00090-n.

Abstract

The development of an effective vaccine is recognised as a necessary adjunct to the control of schistosomiasis japonica, a disease affecting several million people in China and the Philippines. Currently, recombinant Schistosoma japonicum molecules are considered most suitable for large scale vaccine production and a number of genes encoding vaccine candidate polypeptides have been cloned and expressed (see Waine et al., 1993a). One of the molecules providing most promise as a vaccine target is paramyosin (Butterworth, 1992), a major structural protein of thick filaments in the muscle of most invertebrates; paramyosin genes have now been cloned from a range of parasitic helminths, including schistosomes (Limberger and McReynolds, 1990; Laclette et al., 1991; Dahmen et al., 1993; Landa et al., 1993; Mühlschlegel et al., 1993, Nara et al., 1994). The cloning and nucleotide sequence of S. Japonicum paramyosin is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Helminth / genetics
  • Genes, Helminth / genetics*
  • Molecular Sequence Data
  • RNA, Helminth / analysis
  • RNA, Messenger / analysis
  • Schistosoma japonicum / genetics*
  • Sequence Analysis, DNA
  • Tropomyosin / chemistry
  • Tropomyosin / genetics*

Substances

  • DNA, Complementary
  • DNA, Helminth
  • RNA, Helminth
  • RNA, Messenger
  • Tropomyosin

Associated data

  • GENBANK/U11825