Abstract
The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD / metabolism
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Antigens, Surface / metabolism
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CD55 Antigens
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CD59 Antigens
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Cloning, Molecular
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DNA / genetics
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Genetic Complementation Test
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Glycosylphosphatidylinositols / biosynthesis*
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HeLa Cells
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Humans
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In Vitro Techniques
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Membrane Glycoproteins / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mice
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Molecular Sequence Data
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Solubility
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Species Specificity
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Thy-1 Antigens
Substances
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Antigens, CD
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Antigens, Surface
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CD55 Antigens
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CD59 Antigens
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Glycosylphosphatidylinositols
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Membrane Glycoproteins
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Membrane Proteins
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Thy-1 Antigens
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phosphatidylinositol glycan-class A protein
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DNA