Abstract
The present study was designed to reexamine the interaction of granulocyte-macrophage colony-stimulating factor (GM-CSF) with endothelial cells (EC) and to investigate the expression of CSF receptor chains in these cells. In agreement with previous data, GM-CSF induced directional migration and, to a lesser degree, proliferation of human umbilical vein EC. When compared to basic fibroblast growth factor, GM-CSF was comparable in terms of chemotactic activity and was substantially less active in terms of proliferation. Binding studies confirmed the presence of receptors for GM-CSF (GM-CSFR) on EC. The expression of the beta chain common to the GM-CSFR, IL-3 receptor, and IL-5 receptor, as well as of the individual alpha chains, was studied by Northern analysis and/or reverse transcription and polymerase chain reaction. EC expressed high levels of the common beta chain transcripts. Expression of the alpha(GM) and alpha(IL-5) chain mRNA was minimal or absent in normal EC, though the transformed ECV304 endothelial cell line had substantial amounts of alpha(GM) chain mRNA. Unexpectedly, EC expressed alpha(IL-3) chain transcripts. IL-3 induced migration of EC across polycarbonate filters, whereas IL-5 was inactive.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Division / drug effects
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Cells, Cultured
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Chemotaxis / drug effects
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
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Humans
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Interleukin-3 / pharmacology
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Interleukin-5 / metabolism
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Kinetics
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Macromolecular Substances
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Mice
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism*
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Receptors, Interleukin*
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Receptors, Interleukin-3 / genetics
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Receptors, Interleukin-3 / metabolism*
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Receptors, Interleukin-5
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Recombinant Proteins / pharmacology
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Transcription, Genetic
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Umbilical Veins
Substances
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Interleukin-3
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Interleukin-5
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Macromolecular Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
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Receptors, Immunologic
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Receptors, Interleukin
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Receptors, Interleukin-3
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Receptors, Interleukin-5
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Recombinant Proteins
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RNA
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Granulocyte-Macrophage Colony-Stimulating Factor