Abstract
c-erbB3 product is moderately expressed in gastric mucosa, especially in parietal cells. Northern blot analysis revealed that 6.2-kb c-erbB3 transcript was expressed in all gastric cancer cell lines examined, and that 1.4-kb c-erbB3 transcript was expressed as highly as 6.2-kb transcript in MKN45 cells. erbB3-S cDNA, corresponding to 1.4-kb c-erbB3 transcript, was cloned by rapid amplification of cDNA ends. Sequence analysis of erbB3-S cDNA showed that this 1.4-kb c-erbB3 mRNA encoded a secreted receptor. Analysis of partial genomic structure of c-erbB3 gene revealed that the exon specific to secreted receptor was identical with the 5' portion of the intron in c-erbB3 gene. c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cell Membrane / metabolism
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Cloning, Molecular
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Exons
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Gene Expression
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Humans
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Introns
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction
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Protein-Tyrosine Kinases / genetics*
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogenes*
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Receptor, ErbB-3
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Stomach Neoplasms
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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DNA, Neoplasm
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Oligodeoxyribonucleotides
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Cell Surface
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Poly A
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RNA
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Protein-Tyrosine Kinases
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Receptor, ErbB-3
Associated data
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GENBANK/D25302
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GENBANK/S61953
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GENBANK/S64630
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GENBANK/S64631
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GENBANK/S64632
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GENBANK/S64635
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GENBANK/S64636
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GENBANK/S64637
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GENBANK/Z19151
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GENBANK/Z19521