Abstract
Phospholipase C-beta 4(PLC-beta 4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-beta 4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-beta 1, human PLC-beta 2, and rat PLC-beta 3, respectively. The amino acid sequence of PLC-beta 4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-beta subtypes, suggesting that the PLC-beta 4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Blotting, Northern
-
Brain / enzymology*
-
Cattle
-
Cerebellum / enzymology
-
Cloning, Molecular
-
DNA
-
Humans
-
Isoenzymes / biosynthesis
-
Isoenzymes / genetics*
-
Molecular Sequence Data
-
Multigene Family*
-
Poly A / genetics
-
Poly A / isolation & purification
-
RNA / genetics
-
RNA / isolation & purification
-
RNA, Messenger
-
Rats
-
Restriction Mapping
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
-
Type C Phospholipases / biosynthesis
-
Type C Phospholipases / genetics*
Substances
-
Isoenzymes
-
RNA, Messenger
-
Poly A
-
RNA
-
DNA
-
Type C Phospholipases
Associated data
-
GENBANK/D16316
-
GENBANK/D31661
-
GENBANK/D37827
-
GENBANK/L16877
-
GENBANK/L16878
-
GENBANK/L18962
-
GENBANK/L19108
-
GENBANK/L19109
-
GENBANK/L19111
-
GENBANK/L19112