Abstract
Sequences specific for cyclic nucleotide-gated channels (CNG channels) have been amplified by PCR from cDNA of heart, aorta, sino-atrial node, cerebellum, C-cells and kidney. The complete amino acid sequence of a CNG channel from rabbit aorta has been deduced by cloning and sequence analysis of the cDNA. Synthetic RNA derived from this cDNA induces the formation of a functional CNG channel in Xenopus oocytes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Aorta / chemistry*
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Base Sequence
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Cattle
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Codon
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Cyclic AMP / pharmacology*
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Cyclic GMP / pharmacology*
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DNA / chemistry
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DNA / genetics
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Electrophysiology
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Gene Expression*
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Humans
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Ion Channel Gating / drug effects*
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Ion Channels / chemistry*
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Ion Channels / genetics
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Ion Channels / physiology
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Molecular Sequence Data
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Open Reading Frames
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Polymerase Chain Reaction
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Rabbits
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Sequence Homology, Amino Acid
Substances
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Codon
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Ion Channels
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DNA
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Cyclic AMP
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Cyclic GMP