Abstract
A search for POU domain transcription factors in human retina cDNA has led to the identification of Brn-3b, a class IV POU domain protein. Immunohistochemical experiments show that chicken, mouse, rabbit, monkey, and human retinas contain Brn-3b exclusively within a subpopulation of ganglion cells. In the adult mouse brain, Brn-3b is found only within cells in the deep layers of the superior colliculus, in the dorsal periaqueductal gray, and in a small cluster of cells in the brain stem near the area postrema. During the immediate postnatal period, cells containing Brn-3b are distributed in a number of regions within the brain stem and cerebellum. These data suggest that Brn-3b plays a role in determining and/or maintaining the identities of a small number of neurons, including a subset of visual system neurons.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Blotting, Western
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Brain / metabolism
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Cattle
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Cell Line
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Chromosome Mapping
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Cloning, Molecular
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DNA Primers
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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Gene Expression*
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Gene Library
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Humans
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Hybrid Cells
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Immunohistochemistry
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Mice
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Molecular Sequence Data
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Organ Specificity
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Poly A / analysis
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Poly A / metabolism
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Polymerase Chain Reaction / methods
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RNA / analysis
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RNA / metabolism
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RNA, Messenger
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Rats
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Restriction Mapping
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Retinal Ganglion Cells / cytology
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Retinal Ganglion Cells / metabolism*
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Sequence Homology, Amino Acid
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Transcription Factor Brn-3
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Transcription Factor Brn-3B
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Vertebrates
Substances
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DNA Primers
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DNA-Binding Proteins
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POU4F2 protein, human
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RNA, Messenger
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Transcription Factor Brn-3
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Transcription Factor Brn-3B
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Transcription Factors
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Poly A
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RNA