Abstract
The C-nitroso compound 3-nitrosobenzamide, which has been shown to remove zinc from the retroviral-type zinc finger of p7NC nucleocapsid proteins, inhibits acute infection of human immunodeficiency virus type 1 in cultured human lymphocytes. The attachment of the virus to lymphocytes and the activities of critical viral enzymes, such as reverse transcriptase, protease, and integrase, are not affected by 3-nitrosobenzamide. However, the process of reverse transcription to form proviral DNA is effectively abolished by the drug, identifying the mode of action of 3-nitrosobenzamide as interrupting the role of p7NC in accurate proviral DNA synthesis during the infectious phase of the virus life cycle.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antiviral Agents*
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Benzamides / pharmacology*
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Capsid Proteins*
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DNA Nucleotidyltransferases / metabolism
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DNA, Viral / biosynthesis
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Gene Products, gag / chemistry*
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HIV Infections / drug therapy
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HIV Protease / metabolism
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HIV Reverse Transcriptase
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HIV-1 / drug effects*
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HIV-1 / growth & development
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Humans
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Integrases
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Nitroso Compounds / pharmacology*
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Proviruses / genetics
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RNA-Directed DNA Polymerase / metabolism
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Viral Proteins*
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Virus Replication
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Zinc / chemistry
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Zinc Fingers*
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gag Gene Products, Human Immunodeficiency Virus
Substances
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Antiviral Agents
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Benzamides
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Capsid Proteins
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DNA, Viral
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Gene Products, gag
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NCP7 protein, Human immunodeficiency virus 1
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Nitroso Compounds
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Viral Proteins
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gag Gene Products, Human Immunodeficiency Virus
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3-nitrosobenzamide
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DNA Nucleotidyltransferases
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Integrases
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase
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HIV Protease
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Zinc