Xenopus PKN: cloning and sequencing of the cDNA and identification of conserved domains

H Mukai; K Mori; H Takanaga; M Kitagawa; H Shibata; M Shimakawa; M Miyahara; Y Ono

doi:10.1016/0167-4781(95)00030-k

Xenopus PKN: cloning and sequencing of the cDNA and identification of conserved domains

Biochim Biophys Acta. 1995 Apr 4;1261(2):296-300. doi: 10.1016/0167-4781(95)00030-k.

Authors

H Mukai¹, K Mori, H Takanaga, M Kitagawa, H Shibata, M Shimakawa, M Miyahara, Y Ono

Affiliation

¹ Department of Biology, Faculty of Science, Kobe University, Japan.

PMID: 7711077
DOI: 10.1016/0167-4781(95)00030-k

Abstract

cDNA clone encoding Xenopus laevis PKN has been isolated from Xenopus kidney library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 901 amino acids. Immunoprecipitate from cytoplasmic fraction of COS7 cells transfected with this cDNA construct using antiserum against bacterially expressed Xenopus PKN revealed arachidonic acid-dependent autophosphorylation activity. Comparison of the closely related sequences of human and rat PKN with a protein from evolutionarily distant Xenopus, revealed several highly invariant domains in the NH2-terminal regulatory regions, suggesting that they participate in binding interaction with arachidonic acid.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Conserved Sequence
DNA, Complementary / biosynthesis
DNA, Complementary / chemistry*
Molecular Sequence Data
Open Reading Frames
Protein Kinase C
Protein Serine-Threonine Kinases*
Protein-Tyrosine Kinases / chemistry
Protein-Tyrosine Kinases / genetics*
Sequence Alignment
Xenopus / genetics*

Substances

DNA, Complementary
protein kinase N
Protein-Tyrosine Kinases
Protein Serine-Threonine Kinases
Protein Kinase C

Associated data

GENBANK/D43890