Design of a potent and orally active nonpeptide platelet fibrinogen receptor (GPIIb/IIIa) antagonist

Bioorg Med Chem. 1994 Sep;2(9):897-908. doi: 10.1016/s0968-0896(00)82039-8.

Abstract

The direct design of the potent nonpeptide platelet fibrinogen receptor (GPIIb/IIIa) antagonist, 8-[[[4- (aminoiminomethyl)phenyl]amino]carbonyl]-2,3,4,5-tetrahydro-3-oxo- 4- (2-phenylethyl)-1H-1,4-benzodiazepine-2-acetic acid, (3) (SB 207448), based on the structure and conformation of the potent and highly constrained cyclic peptide antagonist SK&F 107260 (2), has been reported [Ku et al., J. Am. Chem. Soc. 1993, 115, 8861]. While 3 displayed in vivo activity in the conscious dog following intravenous administration, it was not active following intraduodenal administration; activity was measured with an ex vivo platelet aggregation assay. The secondary amide in 3 was N-methylated in the expectation of increased absorption and bioavailability. The resulting tertiary amide, 4 (SB 208651), also showed high binding affinity for human GPIIb/IIIa and potent antiaggregatory activity in human platelet-rich plasma. Most importantly, 4 was active in vivo following intravenous and intraduodenal administration. Comparison of the iv and id inhibition curves suggests an apparent bioavailability of approximately 10%. Thus, 4 represents the first orally active compound in this series of potent, nonpeptide fibrinogen receptor antagonists.

MeSH terms

  • Administration, Oral
  • Amino Acid Sequence
  • Animals
  • Benzodiazepinones / chemical synthesis*
  • Benzodiazepinones / pharmacology*
  • Blood Platelets / chemistry*
  • Blood Platelets / ultrastructure
  • Dogs
  • Humans
  • Infusions, Intravenous
  • Kinetics
  • Male
  • Methylation
  • Molecular Sequence Data
  • Molecular Structure
  • Peptides / chemical synthesis
  • Peptides / pharmacology
  • Peptides, Cyclic / metabolism
  • Peptides, Cyclic / pharmacology
  • Platelet Aggregation Inhibitors / chemical synthesis*
  • Platelet Aggregation Inhibitors / metabolism
  • Platelet Aggregation Inhibitors / pharmacology*
  • Platelet Membrane Glycoproteins / antagonists & inhibitors*
  • Platelet Membrane Glycoproteins / metabolism
  • Rabbits

Substances

  • Benzodiazepinones
  • Peptides
  • Peptides, Cyclic
  • Platelet Aggregation Inhibitors
  • Platelet Membrane Glycoproteins
  • SB 208651
  • SK&F 107260