IgM antibody capture haemadherence test (MACHAT) for the detection of measles specific IgM

J Virol Methods. 1994 Dec;50(1-3):167-73. doi: 10.1016/0166-0934(94)90173-2.

Abstract

An antibody capture haemadherence test (MACHAT) for detecting measles-specific IgM is described. The assay is based on the antibody capture principle with rhesus monkey erythrocytes as detector system in place of labelled antisera. MACHAT was compared with a commercial indirect enzyme immunoassay (EIA) for measles-specific IgM using 382 sera from patients notified as measles. There was good agreement between the two tests; 106 sera were found to contain measles IgM by both tests, 7 further sera were positive only in the commercial EIA and 9 only in MACHAT. One sera gave an equivocal result in MACHAT and another in the commercial EIA. Twelve of the 18 sera with discrepant results were also tested by MACRIA; in 7 MACRIA gave the same results as MACHAT, in 3 the MACRIA results agreed with the commercial test and in 2 the MACRIA results were equivocal. Specificity was established by a lack of MACHAT reactivity in sera collected from blood donors (n = 83) and from cases of recent rubella, dengue and parvovirus B19 infection (n = 51). The MACHAT is a simple, cheap test that can be read by eye and is suitable for measles surveillance programmes in the developing world.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Hemadsorption Inhibition Tests*
  • Humans
  • Immunoglobulin M / blood*
  • Measles / diagnosis
  • Measles / immunology
  • Measles / virology*
  • Measles virus / immunology*

Substances

  • Immunoglobulin M