Development of bone marrow immunoscintigraphy using a Tc-99m labeled anti-NCA-95 monoclonal antibody

C W Choi; J K Chung; D S Lee; M C Lee; H K Chung; B K Kim; C S Koh

doi:10.1016/0969-8051(94)e0069-u

Development of bone marrow immunoscintigraphy using a Tc-99m labeled anti-NCA-95 monoclonal antibody

Nucl Med Biol. 1995 Jan;22(1):117-23. doi: 10.1016/0969-8051(94)e0069-u.

Authors

C W Choi¹, J K Chung, D S Lee, M C Lee, H K Chung, B K Kim, C S Koh

Affiliation

¹ Department of Internal Medicine, Seoul National University College of Medicine, Korea.

PMID: 7735161
DOI: 10.1016/0969-8051(94)e0069-u

Abstract

We evaluated the monoclonal antibody CEA-79.4 against carcinoembryonic antigen as an immunoscintigraphic agent for assessing the state of the bone marrow. Western blotting of human granulocyte extracts with the antibody could confirm that the binding was with the epitope of NCA-95. Immunocytochemical staining of bone marrow aspirates revealed specific uptake of this antibody by granulopoietic cells. The affinity constant was 2-9 x 10(9) L/mol. Immunoscintigraphy using 99mTc-labeled CEA-79.4 in a normal volunteer revealed high uptake in the bone marrow as compared to other organs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal*
Antigens, Neoplasm*
Bone Marrow / diagnostic imaging*
Carcinoembryonic Antigen / immunology
Cell Adhesion Molecules*
Humans
Membrane Glycoproteins / immunology*
Radioimmunodetection*
Technetium*

Substances

Antibodies, Monoclonal
Antigens, Neoplasm
Carcinoembryonic Antigen
Cell Adhesion Molecules
Membrane Glycoproteins
Technetium