Immune complex specific for the pancreatic duct antigen in patients with idiopathic chronic pancreatitis and Sjögren syndrome

Autoimmunity. 1994;19(1):23-9. doi: 10.3109/08916939409008005.

Abstract

Circulating immune complexes (ICs) containing the pancreatic antigen against SP3-1 monoclonal antibody were measured in patients with idiopathic chronic pancreatitis (ICP) and Sjögren syndrome (SjS) by Raji cell and solid-phase radioimmuno-assays (RIA). The mean serum levels of ICs measured by solid-phase RIA were significantly higher in patients with ICP (n = 23) and SjS (n = 21) than control (n = 15, p < 0.05, p < 0.02, respectively). ICs were positive in 10 patients with ICP (43%) and 12 SjS patients (57%). Raji cell assay also revealed a significantly higher serum ICs levels in patients with ICP (n = 17) and SjS (n = 12) compared with those of control (n = 7, p < 0.025, p < 0.005, respectively). Seven patients with ICP (41%) and 8 SjS patients (67%) had positive ICs. This was in contrast to the normal level of ICs in patients with alcoholic chronic pancreatitis, primary biliary cirrhosis, and chronic thyroiditis. Our analysis demonstrated a significant and positive relationship between RIA and Raji cell assay (r = 0.70, p < 0.05). Our results suggest that ICs specific for SP3-1 may play a role in the pathophysiology of ICP and SjS.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcoholism / complications
  • Antibody Specificity
  • Antigen-Antibody Complex / blood*
  • Autoantibodies / blood*
  • Autoantibodies / immunology
  • Autoantigens / immunology*
  • Autoimmune Diseases / immunology*
  • Burkitt Lymphoma / pathology
  • Cholelithiasis / complications
  • Chronic Disease
  • Fluorescent Antibody Technique
  • Humans
  • Pancreatic Ducts / immunology*
  • Pancreatitis / etiology
  • Pancreatitis / immunology*
  • Radioimmunoassay
  • Reproducibility of Results
  • Sjogren's Syndrome / immunology*
  • Thyroiditis / immunology
  • Tumor Cells, Cultured

Substances

  • Antigen-Antibody Complex
  • Autoantibodies
  • Autoantigens