Abstract
A full-length Arabidopsis thaliana cDNA (app) encoding a protein with high similarity (about 60%) to the catalytic domain of vertebrate poly(ADP-ribose) polymerase (PARP; EC 2.4.2.30) has been cloned. The N-terminal extension of the Arabidopsis protein shows similarities with domains of different nuclear and DNA binding proteins in agreement with nuclear localization and putative function of a plant PARP. APP is encoded by a single gene mapped at the top of chromosome 4 of the Arabidopsis genome and mRNA is abundant in cell suspension culture compared to its accumulation in whole plant.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arabidopsis / enzymology*
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Arabidopsis / genetics*
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA, Complementary / genetics*
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DNA, Plant / genetics*
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Genes, Plant
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Humans
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Molecular Sequence Data
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Phylogeny
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Poly(ADP-ribose) Polymerases / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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DNA, Plant
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RNA, Messenger
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RNA, Plant
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Poly(ADP-ribose) Polymerases