Abstract
Immunoglobulin M (IgM) and IgG1 monoclonal antibody isotype switch variants to Cryptococcus neoformans capsular polysaccharide were used to study the sensitivity of a double sandwich enzyme-linked immunosorbent assay (ELISA). The most sensitive ELISA configurations used IgG1 monoclonal antibody absorbed on polystyrene plates or IgM immobilized with goat antisera for antigen capture.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Fungal / immunology*
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Antibodies, Monoclonal / immunology
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Antigens, Fungal / analysis*
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Cryptococcus neoformans / immunology*
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Cryptococcus neoformans / isolation & purification
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Enzyme-Linked Immunosorbent Assay / methods*
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Goats
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Immunoglobulin G / immunology
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Immunoglobulin M / immunology
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Mice
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Mice, Inbred BALB C
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Sensitivity and Specificity
Substances
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Antibodies, Fungal
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Antibodies, Monoclonal
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Antigens, Fungal
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Immunoglobulin G
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Immunoglobulin M