Abstract
A voltage-sensitive inwardly rectifying chloride (Cl-) conductance (GCl(V) is present in hippocampal pyramidal but not dentate gyrus neurons and has a significant role in modulation of neuronal inhibition by GABA. GCl(V) has the same activation properties as the cloned and expressed Cl- channel CIC-2. In brain, CIC-2 was detected selectively in neurons, and in hippocampus was detected in the same populations of neurons that demonstrate GCl(V). CIC-2 mRNA expression varied widely in different neuronal populations in brain but was greatest in pyramidal and other large neurons and least in interneurons. The observed differential expression of CIC-2 provides a potential molecular basis for the paradoxical excitation produced by GABAA receptor activation in selected neuronal populations.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Brain / metabolism
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Brain / physiology*
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CLC-2 Chloride Channels
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Cell Communication
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Chloride Channels / biosynthesis*
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Chloride Channels / physiology
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DNA Primers
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Electric Conductivity
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Gene Expression*
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Interneurons / physiology
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Membrane Potentials
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Molecular Sequence Data
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Nerve Tissue Proteins / biosynthesis*
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Neurons / drug effects
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Neurons / metabolism
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Neurons / physiology*
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Oligonucleotide Probes
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Organ Specificity
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Polymerase Chain Reaction
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Pyramidal Cells / physiology
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Spinal Cord / physiology
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Synapses / drug effects
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Synapses / physiology*
Substances
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CLC-2 Chloride Channels
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Chloride Channels
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DNA Primers
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Nerve Tissue Proteins
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Oligonucleotide Probes
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RNA, Messenger