Abstract
We have developed a system for studying hepatocellular growth potential in which liver cells are introduced into the diseased livers of albumin-urokinase (Alb-uPA) transgenic mice. To use this system to study xenogeneic cell transplantation, rat liver cells were introduced into immunotolerant Alb-uPA transgenic mice. In regenerated recipient livers, up to 100% of hepatocellular gene expression was of rat origin, demonstrating the creation of a functional mouse liver in which parenchyma is derived from xenogeneic (rat) hepatocytes. Immunotolerant Alb-uPA transgenic mice provide a tool for studying hepatocellular biology of any species, including humans, in a controlled experimental setting.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Transplantation*
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DNA / analysis
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Female
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Immunohistochemistry
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Liver / cytology*
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Liver / physiology
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Liver Regeneration*
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Male
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Mice
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Mice, Nude
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Mice, Transgenic
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Molecular Sequence Data
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Oligonucleotide Probes
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RNA, Messenger / analysis
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Rats
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Rats, Sprague-Dawley
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Recombinant Fusion Proteins / biosynthesis
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Serum Albumin / biosynthesis
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Serum Albumin / genetics
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Transferrin / analysis
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Transferrin / biosynthesis
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Transplantation, Heterologous*
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Urokinase-Type Plasminogen Activator / biosynthesis
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Urokinase-Type Plasminogen Activator / genetics
Substances
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Oligonucleotide Probes
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RNA, Messenger
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Recombinant Fusion Proteins
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Serum Albumin
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Transferrin
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DNA
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Urokinase-Type Plasminogen Activator