Abstract
The cytotoxic necrotizing factor type 1 (CNF1) from E. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 with the cell cytoskeleton probably promotes the cell spreading and interferes with the cytokinesis, leading to the formation of giant multinucleated cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Animals
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Bacterial Toxins / toxicity*
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Biotechnology
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Cell Line
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Cytoskeleton / drug effects*
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Cytoskeleton / metabolism
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Cytotoxins / toxicity*
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Escherichia coli Proteins*
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Giant Cells / drug effects
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Giant Cells / metabolism
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Giant Cells / ultrastructure
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Humans
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Necrosis
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Rabbits
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Skin / drug effects
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Skin / pathology
Substances
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Actins
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Bacterial Toxins
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Cytotoxins
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Escherichia coli Proteins
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cytotoxic necrotizing factor type 1