Abstract
C alpha-dehydrogenase catalyzes the oxidation of arylglycerol-beta-aryl ether at the C alpha-position, and therefore this process produces the specific substrate for beta-etherase, which cleaves beta-ary ethers (C alpha carbonyl type). Here we isolated the C alpha-dehydrogenase gene (lig D) and sequenced its nucleotides. This gene contains an open reading frame of 915 bp and the deduced amino acid sequence had a homology with the ribitol dehydrogenase family. lig D is about 1 kbp upstream of the beta-etherase gene (lig E).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / chemistry
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Blotting, Southern
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Catalysis
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Cloning, Molecular
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Genes, Bacterial
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Lignin / metabolism*
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Molecular Sequence Data
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Oxidation-Reduction
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Oxidoreductases / chemistry
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Oxidoreductases / genetics*
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Oxidoreductases / metabolism
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Pseudomonas / enzymology*
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Pseudomonas / genetics
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Restriction Mapping
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Sequence Alignment
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Sequence Homology, Amino Acid
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Substrate Specificity
Substances
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Bacterial Proteins
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Lignin
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Oxidoreductases
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aryl ether cleaving enzyme
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Alcohol Oxidoreductases
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Calpha-dehydrogenase