Inhibition of the endothelin-converting enzyme by pepsin digests of food proteins

M Okitsu; A Morita; M Kakitani; M Okada; H Yokogoshi

doi:10.1271/bbb.59.325

Inhibition of the endothelin-converting enzyme by pepsin digests of food proteins

Biosci Biotechnol Biochem. 1995 Feb;59(2):325-6. doi: 10.1271/bbb.59.325.

Authors

M Okitsu¹, A Morita, M Kakitani, M Okada, H Yokogoshi

Affiliation

¹ School of Food and Nutritional Sciences, University of Shizuoka, Japan.

PMID: 7766033
DOI: 10.1271/bbb.59.325

Abstract

Inhibitory activities toward the endothelin-converting enzyme (ECE) were detected in pepsin digests of bonito pyrolic appendix and beef. After Sep-Pak C18 fractionation, this activity from bonito and beef was recovered in the 50% and 25% ethanol fractions, respectively. Activities were also recovered in the ultrafiltrate (< 5000 Da), and disappeared after a pronase treatment. Therefore, these inhibitory activities may have peptidic properties.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aspartic Acid Endopeptidases / antagonists & inhibitors*
Cattle
Chemical Fractionation
Dietary Proteins / metabolism*
Endothelin-Converting Enzymes
Ethanol / chemistry
Food Handling
Meat
Metalloendopeptidases / antagonists & inhibitors*
Pepsin A / metabolism*
Pronase / metabolism
Tuna
Ultrafiltration

Substances

Dietary Proteins
Ethanol
Aspartic Acid Endopeptidases
Pepsin A
Metalloendopeptidases
Pronase
Endothelin-Converting Enzymes