Abstract
We investigated whether somatic rearrangements in minisatellite DNA are more frequent in chemically induced mouse liver tumors than they are in spontaneous tumors. CD-1 mouse liver tumors were induced by either a single dose or 15 consecutive daily doses of 7,12-dimethylbenz[alpha]anthracene, 4-aminoazobenzene, N-hydroxy-2-acetyl-aminofluorene or diethylnitrosoamine (DEN). Using DNA fingerprinting analysis, we found that the single- and multiple-dose carcinogen treatments caused a 2- to 5-fold higher frequency of minisatellite DNA rearrangements compared with that found in spontaneous tumors--with the exception of single-dose DEN tumors, which showed no increase in rearrangements. Our results suggest that DNA fingerprinting may be a valuable assay for differentiating certain chemically induced tumors from spontaneous tumors.
MeSH terms
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9,10-Dimethyl-1,2-benzanthracene / toxicity
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Adenoma / chemically induced
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Adenoma / genetics
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Adenoma / metabolism
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Adenoma / pathology
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Animals
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Carcinogens / toxicity*
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Carcinoma / chemically induced
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Carcinoma / genetics
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Carcinoma / metabolism
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Carcinoma / pathology
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DNA Fingerprinting
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DNA, Neoplasm / isolation & purification
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DNA, Neoplasm / metabolism
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DNA, Satellite / drug effects*
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DNA, Satellite / isolation & purification
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DNA, Satellite / metabolism
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Diethylnitrosamine / toxicity
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Hydroxyacetylaminofluorene / toxicity
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Liver Neoplasms / chemically induced
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism*
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Liver Neoplasms / pathology
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Mice
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Mice, Inbred Strains
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p-Aminoazobenzene / toxicity
Substances
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Carcinogens
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DNA, Neoplasm
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DNA, Satellite
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Diethylnitrosamine
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Hydroxyacetylaminofluorene
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9,10-Dimethyl-1,2-benzanthracene
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p-Aminoazobenzene