Abstract
Mutants of Saccharomyces cerevisiae without phosphoenolpyruvate carboxykinase activity showed no measurable lactate proton symport, while mutants without fructose-1,6-bisphosphatase had normal transport activity. Incubation of a pck1 mutant, under derepression conditions in the presence of glycerol, restored the activity of the lactate-proton symport, with identical kinetic characteristics to that in the wild-type. For efficient lactate-proton symport activity, not only is an external inducer such as lactic acid needed, but also a molecule derived from the acid metabolism may be necessary.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Fructose-Bisphosphatase / genetics
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Fructose-Bisphosphatase / metabolism
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Gene Expression Regulation, Fungal
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Ion Transport / genetics*
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Lactates / metabolism*
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Lactic Acid
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Mutagenesis
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Phosphoenolpyruvate Carboxykinase (GTP) / genetics
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Phosphoenolpyruvate Carboxykinase (GTP) / metabolism*
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Protons
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Restriction Mapping
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
Substances
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Carrier Proteins
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Lactates
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Protons
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Lactic Acid
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Fructose-Bisphosphatase
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Phosphoenolpyruvate Carboxykinase (GTP)