Abstract
The CCK-8 analogue, SNF 8702, was radioiodinated. [125I]SNF 8702 showed high-affinity specific binding for both guinea pig and mouse brain tissues, whereas no specific binding was seen for guinea pig pancreatic tissue. The properties of the site labeled by [125I]SNF 8702 were characterized by binding inhibition studies for a series of CCKA and CCKB receptor ligands. The binding selectivity profile corresponded to that for the CCKB receptor. The labeled compound is stable for more than 6 weeks during storage at -20 degrees C.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Brain / metabolism*
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Cell Membrane / metabolism
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Guinea Pigs
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Iodine Radioisotopes
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Isotope Labeling / methods
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Mice
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Molecular Sequence Data
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Organ Specificity
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Pancreas / metabolism
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Peptide Fragments / metabolism*
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Radioligand Assay
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Receptors, Cholecystokinin / analysis
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Receptors, Cholecystokinin / metabolism*
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Sincalide / analogs & derivatives*
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Sincalide / metabolism
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Species Specificity
Substances
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Iodine Radioisotopes
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Peptide Fragments
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Receptors, Cholecystokinin
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SNF 8702
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Sincalide