Functional distinction among structural subsections in the specific priming signal for DNA replication of the broad host-range plasmid RSF1010

Biosci Biotechnol Biochem. 1995 May;59(5):920-1. doi: 10.1271/bbb.59.920.

Abstract

To analyze the functional contribution to the ssiA function of subsections of the ssiA-determinant sequence based on their dimensions, we constructed ssiA mutants carrying insertions and deletions. Results of the examination of the ssiA mutants told us that, in addition to the base sequence, the dimensions were crucial factors for the functional contribution of the subsections of ssiA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Base Sequence
  • Cell Division
  • DNA Replication / genetics*
  • DNA, Recombinant
  • Escherichia coli / genetics
  • Genes, Dominant
  • Genetic Vectors
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • R Factors / genetics*
  • Subtilisins / genetics
  • Transfection

Substances

  • Bacterial Proteins
  • DNA, Recombinant
  • Subtilisins

Associated data

  • GENBANK/M28829