Abstract
The carboxyl-terminal fragment of CENP-B contains a major epitope for anti-centromere antibodies (ACA). We have developed an enzyme-linked immunoassay (ELISA) for measuring antibodies to the 147-carboxyl-terminal amino acids of CENP-B expressed as a beta-galactosidase fusion protein. The ELISA was 98% sensitive and 95% specific for detecting ACA in a population which included 46 patients with ACA detected by other means. Therefore, the CENP-B ELISA should prove a valuable tool in screening for ACA in populations at risk of developing systemic sclerosis, such as those with Raynaud's phenomenon. Levels of anti-CENP-B antibodies were not increased in unaffected relatives of probands with ACA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Autoantibodies / blood*
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Autoantigens*
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Autoimmune Diseases / immunology*
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Centromere / immunology*
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Centromere Protein B
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Chromosomal Proteins, Non-Histone / immunology*
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DNA-Binding Proteins / immunology*
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunodominant Epitopes / immunology
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Liver Cirrhosis, Biliary / immunology
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Lupus Erythematosus, Systemic / immunology
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Peptide Fragments / immunology
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Scleroderma, Systemic / immunology
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Sensitivity and Specificity
Substances
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Autoantibodies
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Autoantigens
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CENPB protein, human
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Centromere Protein B
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Immunodominant Epitopes
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Peptide Fragments