G-protein gamma 7 subunit is selectively expressed in medium-sized neurons and dendrites of the rat neostriatum

J Neurosci Res. 1994 Sep 1;39(1):108-16. doi: 10.1002/jnr.490390113.

Abstract

We used subtractive hybridization to isolate clones of gamma 7, a 68 residue G-protein gamma subunit. Northern blotting and in situ hybridization reveal that the gamma 7 subunit mRNA is expressed primarily in medium-sized neurons of the neostriatum and nucleus accumbens and neurons of the olfactory tubercle, and at low levels in the dentate gyrus of the hippocampal formation and laminae II-III, and V of the neocortex. The gamma 7 mRNA is translocated into dendrites of neurons in the neostriatum and the dentate gyrus of the hippocampus. gamma 7 is expressed at relatively very low concentrations in peripheral tissues. The selective pattern of gamma 7 expression within the brain is highly reminiscent of those of the striatum-enriched adenylyl cyclase ACST, dopamine receptors, and the alpha subunit of G(olf), suggesting that, in striatum, gamma 7 may be a subunit of a G(olf) alpha-containing G protein that couples dopamine receptors selectively to ACST.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Size
  • DNA, Complementary / genetics
  • Dendrites / metabolism
  • Digoxigenin
  • GTP-Binding Proteins / biosynthesis*
  • GTP-Binding Proteins / genetics
  • Gene Expression Regulation*
  • Hippocampus / cytology
  • Hippocampus / metabolism
  • Male
  • Mice
  • Molecular Sequence Data
  • Neostriatum / cytology
  • Neostriatum / metabolism*
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Neurons / metabolism*
  • Nucleic Acid Hybridization
  • Nucleus Accumbens / cytology
  • Nucleus Accumbens / metabolism
  • Olfactory Pathways / cytology
  • Olfactory Pathways / metabolism
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, Dopamine
  • GTP-Binding Proteins
  • Adenylyl Cyclases
  • Digoxigenin

Associated data

  • GENBANK/L23219