Abstract
The 5'-flanking region of the gene encoding human muscle glycogen synthase was isolated from a human placental genomic library and sequenced. The sequence is TATA-less and G+C-rich, and putative transcription-controlling sequences were identified. Furthermore, a simple (dC-dA)n sequence repeat was identified about 4 kb upstream from the start codon. This sequence was highly polymorphic and five alleles were typed in the Japanese population using the polymerase chain reaction.
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cloning, Molecular
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DNA / genetics
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DNA / metabolism
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DNA Primers
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DNA, Satellite / genetics
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Female
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Genomic Library
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Glycogen Synthase / biosynthesis
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Glycogen Synthase / genetics*
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Hominidae / genetics*
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Humans
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Molecular Sequence Data
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Muscles / enzymology*
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Placenta / enzymology
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Polymerase Chain Reaction
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Polymorphism, Genetic
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Pregnancy
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Promoter Regions, Genetic*
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Rats
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Repetitive Sequences, Nucleic Acid
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TATA Box
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Transcription Factors / metabolism
Substances
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DNA Primers
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DNA, Satellite
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Transcription Factors
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DNA
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Glycogen Synthase