Combined immunophenotyping and karyotyping in peripheral T cell lymphomas demonstrating different clonal and nonclonal chromosome aberrations in T helper cells

Leuk Lymphoma. 1994 Sep;15(1-2):113-25. doi: 10.3109/10428199409051685.

Abstract

Combined immunophenotyping and karyotyping was performed in seven cases of peripheral T cell lymphoma with complex aberrant clones. Various lymphocytic cell populations entered mitosis, whereas all aberrant cells belonged to the T helper/inducer cell population. Lymphomas with the same recurrent chromosome aberrations, i.e. inversion inv(14)(q11q32.1) and isochromosome i(8)(q10), had a very similar immunophenotype. The aberrant cells in these cases expressed CD3+, CD4+, CD7+, CD45RO+. The immunophenotypic similarity is underlined in one case of T prolymphocytic leukemia, in whom the aberrant cells lost the CD8 antigen originally present, during cultivation with PHA. In one case of Sézary's syndrome, two or possibly even three different clones as well as nonclonal aberrations were identified within the T (helper/inducer) cell population, providing further evidence that chromosomal instability is a characteristic feature of cutaneous T cell lymphoma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antigens, CD / analysis
  • Antigens, Neoplasm / analysis
  • Chromosome Aberrations*
  • Clone Cells / immunology
  • Clone Cells / pathology
  • Female
  • Humans
  • Immunophenotyping*
  • Karyotyping*
  • Leukemia, Prolymphocytic / genetics
  • Leukemia, Prolymphocytic / pathology*
  • Leukemia, Prolymphocytic, T-Cell / genetics
  • Leukemia, Prolymphocytic, T-Cell / pathology*
  • Lymphoma, Non-Hodgkin / genetics
  • Lymphoma, Non-Hodgkin / pathology*
  • Lymphoma, T-Cell, Peripheral / genetics
  • Lymphoma, T-Cell, Peripheral / pathology*
  • Male
  • Middle Aged
  • Neoplastic Stem Cells / immunology
  • Neoplastic Stem Cells / pathology*
  • Sezary Syndrome / genetics
  • Sezary Syndrome / pathology*
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Helper-Inducer / pathology*

Substances

  • Antigens, CD
  • Antigens, Neoplasm