Polymerase chain reaction detection of two novel human N-acetylgalactosamine-6-sulfate sulfatase gene polymorphisms by single-strand conformation polymorphism analysis or by StyI and StuI cleavages

S Tomatsu; S Fukuda; A Uchiyama; T Hori; Y Nakashima; K Sukegawa; N Kondo; Y Suzuki; N Shimozawa; T Orii

doi:10.1007/BF00209415

Polymerase chain reaction detection of two novel human N-acetylgalactosamine-6-sulfate sulfatase gene polymorphisms by single-strand conformation polymorphism analysis or by StyI and StuI cleavages

Hum Genet. 1995 Feb;95(2):243-4. doi: 10.1007/BF00209415.

Authors

S Tomatsu¹, S Fukuda, A Uchiyama, T Hori, Y Nakashima, K Sukegawa, N Kondo, Y Suzuki, N Shimozawa, T Orii

Affiliation

¹ Department of Pediatrics, Gifu University School of Medicine, Japan.

PMID: 7860078
DOI: 10.1007/BF00209415

Abstract

We describe two common single-base polymorphisms of the N-acetylgalactosamine-6-sulfate sulfatase gene after StyI or StuI restriction sites. These polymorphisms were readily detected by single-strand conformation polymorphism (SSCP), using the polymerase chain reaction (PCR).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chondroitinsulfatases / genetics*
Deoxyribonucleases, Type II Site-Specific
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic*
Polymorphism, Single-Stranded Conformational

Substances

endodeoxyribonuclease StyI
AGGCCT-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific
Chondroitinsulfatases