Neurospora crassa blue light-inducible gene bli-3

Biochem Mol Biol Int. 1994 Oct;34(4):737-44.

Abstract

Blue light induces various physiological, morphological and biochemical reactions in the filamentous fungus Neurospora crassa. This light response is accompanied by a global change in gene expression, and several light-inducible transcripts (bli-genes) have been cloned. We isolated the genomic clone of the gene bli-3, whose mRNA we have previously shown to be induced 2 minutes after the beginning of illumination. Its DNA sequence predicted a transcriptional unit of 1050 bp encoding a novel, hydrophilic protein of 209 aminoacids. Comparison to other N. crassa genes revealed a group of inducible genes which share this promoter structure: a well conserved TATA-box, similar transcription start box (TCATCANC) and repeats of pyrimidines (CT) between these boxes. Based upon this group, we propose a consensus sequence for one possible type of inducible promoter in N. crassa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carotenoids / metabolism
  • Consensus Sequence
  • DNA, Fungal / chemistry
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Gene Expression / radiation effects*
  • Genes, Fungal*
  • Introns
  • Light*
  • Molecular Sequence Data
  • Neurospora crassa / genetics*
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • Repetitive Sequences, Nucleic Acid
  • TATA Box

Substances

  • BLI-3 protein, Neurospora crassa
  • DNA, Fungal
  • Fungal Proteins
  • Carotenoids

Associated data

  • GENBANK/X81318