Abstract
This article describes the expression pattern and functional analysis of Lazarillo, a novel cell surface glycoprotein expressed in the embryonic grasshopper nervous system, and a member of the lipocalin family. Lazarillo is expressed by a subset of neuroblasts, ganglion mother cells and neurons of the central nervous system, by all sensory neurons of the peripheral nervous system, and by a subset of neurons of the enteric nervous system. It is also present in a few non neuronal cells associated mainly with the excretory system. A monoclonal antibody raised against Lazarillo perturbs the extent and direction of growth of identified commissural pioneer neurons. We propose that Lazarillo is the receptor for a midline morphogen involved in the outgrowth and guidance of these neurons.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Axons / physiology*
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Axons / ultrastructure
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Carrier Proteins / analysis
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Carrier Proteins / genetics*
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Carrier Proteins / physiology
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Cells, Cultured
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Central Nervous System / chemistry
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Central Nervous System / embryology
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Gene Expression Regulation, Developmental*
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Genes, Insect*
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Grasshoppers / embryology*
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Grasshoppers / genetics
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Immunohistochemistry
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In Situ Hybridization
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Insect Proteins*
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Intestines / chemistry
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Intestines / embryology
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Intestines / innervation
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Lipocalins
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Membrane Glycoproteins / analysis
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / physiology
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Nervous System / chemistry
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Nervous System / embryology*
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Nervous System / ultrastructure
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Neurons / chemistry
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Neurons / physiology
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Neurons / ultrastructure
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Peripheral Nervous System / chemistry
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Peripheral Nervous System / embryology
Substances
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Carrier Proteins
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Insect Proteins
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Lazarillo protein, Schistocerca
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Lipocalins
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Membrane Glycoproteins