Genetic stability of protein expression systems in yeast

Dev Biol Stand. 1994:83:13-9.

Abstract

For the expression of recombinant proteins in yeast, genetic stability is monitored using a combination of standard microbiology and nucleic acid testing procedures. Process consistency during the cell amplification and product expression phases of fermentation are also reliable indicators of stability. The potential for instability arising from point mutation, gene conversion or recombination has been shown to occur at a low frequency and does not generally affect protein product quality.

Publication types

  • Review

MeSH terms

  • Biotechnology / standards
  • DNA, Recombinant / genetics
  • Gene Expression
  • Genetic Markers
  • Mutation
  • Phenotype
  • Plasmids / genetics
  • Quality Control
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics*
  • Recombination, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • DNA, Recombinant
  • Genetic Markers
  • Recombinant Proteins